Purification and structure of mutacin B-Ny266: a new lantibiotic produced byStreptococcus mutans
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چکیده
منابع مشابه
Biosynthesis and transport of the lantibiotic mutacin 1140 produced by Streptococcus mutans.
UNLABELLED Lantibiotics are ribosomally synthesized peptide antibiotics composed of an N-terminal leader peptide that is cleaved to yield the active antibacterial peptide. Significant advancements in molecular tools that promote the study of lantibiotic biosynthesis can be used in Streptococcus mutans. Herein, we further our understanding of leader peptide sequence and core peptide structural r...
متن کاملMutacin II, a Bactericidal Lantibiotic from Streptococcus mutans
MICHAEL L. CHIKINDAS,† JAN NOVÁK, ARNOLD J. M. DRIESSEN,* WIL N. KONINGS, KURT M. SCHILLING,‡ AND PAGE W. CAUFIELD Unilever Dental Research, Bebington L63 3JW, United Kingdom; Department of Oral Biology, School of Dentistry, University of Alabama at Birmingham, Birmingham, Alabama 35294; and Department of Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of G...
متن کاملGenetic and biochemical analysis of mutacin 1140, a lantibiotic from Streptococcus mutans.
Streptococcus mutans JH1000 and its derivatives were previously shown (J. D. Hillman, K. P. Johnson, and B. I. Yaphe, Infect. Immun. 44:141-144, 1984) to produce a low-molecular-weight, broad-spectrum bacteriocin-like inhibitory substance (BLIS). The thermosensitive vector pTV1-OK harboring Tn917 was used to isolate a BLIS-deficient mutant, DM25, and the mutated gene was recovered by shotgun cl...
متن کاملIsolation and biochemical characterization of a novel lantibiotic mutacin from Streptococcus mutans.
Certain members of the indigenous biota of humans produce antimicrobial substances called bacteriocins, which inhibit other bacteria, including members of their own species. One of these substances, mutacin, is made by Streptococcus mutans, a member of the oral biota. Mutacin inhibits other mutans streptococci as well as many gram-positive exogenous pathogens. Here, we report for the first time...
متن کاملStructure-activity study of the lantibiotic mutacin II from Streptococcus mutans T8 by a gene replacement strategy.
Mutacin II, elaborated by group II Streptococcus mutans, is a ribosomally synthesized and posttranslationally modified polypeptide antibiotic containing unusual thioether and didehydro amino acids. To ascertain the role of specific amino acid residues in mutacin II antimicrobial activity, we developed a streptococcal expression system that facilitates the replacement of the mutA gene with a sin...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1997
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(97)00425-0